Microvasculature Denoising and Enhancement in Fluorescence Microscopy Images

V. B. Surya Prasath1, Rengarajan Pelapur1, Olga Glinskii2,3, Vladislav Glinsky2,4, Virginia Huxley3,5, K. Palaniappan1
1Computational Imaging and Visualization Analysis Lab, Department of Computer Science
2Department of Medical Pharmacology and Physiology
3Department of Pathology and Anatomical Sciences
4National Center for Gender Physiology
5Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65201 USA

University of Missouri-Columbia, USA


Fluorescence microscopy images are contaminated by noise and improving image quality without blurring vascular structures by filtering is an important step in automatic image analysis. The application of interest here is to automatically extract the structural components of the microvascular system with accuracy from images acquired by fluorescence microscopy. A robust denoising process is necessary in order to extract accurate vascular morphology information. For this purpose, we propose a multiscale tensor with anisotropic diffusion model which progressively and adaptively updates the amount of smoothing while preserving vessel boundaries accurately. Based on a coherency enhancing flow with planar confidence measure and fused 3D structure information, our method integrates multiple scales for microvasculature preservation and noise removal membrane structures. Experimental results on simulated synthetic images and epifluorescence images show the advantage of our improvement over other related diffusion filters. We further show that the proposed multiscale integration approach improves denoising accuracy of different tensor diffusion methods to obtain better microvasculature segmentation.

Restoration Results

More Results:
See the webpage: Denoise which is updated more often than here!


V. B. S. Prasath, R. Pelapur, O. V. Glinskii, V. V. Glinsky, V. H. Huxley, K. Palaniappan. Multi-scale Tensor Anisotropic Filtering of Fluorescence Microscopy for Denoising Microvasculature. IEEE International Symposium on Biomedical Imaging (ISBI), April 2015.

Surya Prasath