#343: DMNet: Dual-stream marker guided deep network for dense cell segmentation and lineage tracking

R. Bao, N. Al-Shakarji, F. Bunyak, and K. Palaniappan

IEEE International Conference on Computer Vision Workshops (ICCVW), pgs. 3354-3363, 2021

training, image segmentation, shape, microscopy, stem cells, cell detection, cell tracking, mitosis

PlainText, Bibtex, PDF, URL, DOI, Google Scholar


Accurate segmentation and tracking of cells in microscopy image sequences is extremely beneficial in clinical diagnostic applications and biomedical research. A continuing challenge is the segmentation of dense touching cells and deforming cells with indistinct boundaries, in low signal-to-noise-ratio images. In this paper, we present a dual-stream marker-guided network (DMNet) for segmentation of touching cells in microscopy videos of many cell types. DMNet uses an explicit cell marker-detection stream, with a separate mask-prediction stream using a distance map penalty function, which enables supervised training to focus attention on touching and nearby cells. For multi-object cell tracking we use M2Track tracking-by-detection approach with multi-step data association. Our M2Track with mask overlap includes short term track-to-cell association followed by track-to-track association to re-link tracklets with missing segmentation masks over a short sequence of frames. Our combined detection, segmentation and tracking algorithm has proven its potential on the IEEE ISBI 2021 6th Cell Tracking Challenge (CTC-6) where we achieved multiple top three rankings for diverse cell types. Our team name is MU-Ba-US, and the implementation of DMNet is available at, http://celltrackingchallenge.net/participants/MU-Ba-US/.